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Keywords 16S rRNA;gene chip;diagnose;bacteria; 基因芯片;诊断;细菌;
Keywords 16S rRNA gene;pulsed field gel electrophoresis;streptococcus mitis; 16S核糖体RNA基因;脉冲场凝胶电泳;缓症链球菌;
Nucleotide sequences of 472~481bp for 16S rRNA gene and 658bp for COI gene were obtained, respectively. 得到的序列总长度分别为472~481bp(16S)和658bp(COI)。
The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
In this study, phylogenetic relationship of 7 Soleoidei species are analysed by sequence variation of both 16S rRNA gene and COI gene. 本文对7种鳎亚目(Soleoidei)鱼类线粒体16S rRNA基因片段和COI基因片段进行了序列比较和分子系统学分析。
Genetic distances between S. grandis and S. strictus were 0.0856 (with 16S rRNA gene) and 0.1712 (with COI gene), respectively. 数据分析结果表明16S rRNA和COI基因片段大竹蛏与长竹蛏两片段的遗传距离分别为0.;0856和0 1712;两竹蛏类与小刀蛏的遗传距离分别为0
Objective To compare the sensitivity and specificity in identifying coagulase negative staphylococcus(CNS) between Vitek32 microbic system and 16S rRNA sequence analysis. 目的比较Vitek32鉴定系统和16S rRNA序列分析法鉴定凝固酶阴性葡萄球菌的灵敏度和特异性。
The 16S rRNA sequence of the screened strain was most closely related to FSB201 and IAM 167 Tetragenococcus halophilus with 99% nucleotide being identical. 进一步对该菌进行分子生物学鉴定,测得菌株的16SrRNA序列与GenBank登记号为FSB201的嗜盐四联球菌(Tetragenococcus halophilus)具有99%25的同源性。
Mitochondrial 16S rRNA and COI gene fragments of Portunus trituberculatus from Weifang were amplified via PCR, then the PCR products were purified and sequenced. 本文采用 PCR扩增和序列测定等技术 ,对三疣梭子蟹 (Portunustrituberculatus)线粒体DNA1 6Sr RNA和 COI基因片段进行了初步研究。
Analysis of 16S rRNA gene sequence indicated that the alkaliphilic strain EMB2039 exhibited maximum similarity with the modal strain DSM 6307~T of Bacillus cohnii. 16 S rDNA测序及系统发育分析结果表明EMB2039与Bacillus cohnii模式菌株DSM 6307~T亲缘关系最近。
On the basis of homology and phylognetic analysis about 16S rRNA gene (16S rDNA), it could be speculated that the strain AB1 is a novel member of the genus Natronococcus. 基于16S rDNA序列的同源性比较及系统发育学研究表明，AB1是Natronococcus属中新成员。
Keywords Vibrio cholerae;Cholerae enterotoxin;16S rRNA gene typing; 霍乱弧菌;霍乱肠毒素;核糖体基因分型;
A multiplex PCR was developed by using 5 sets of primers that identifies the sequences of E coli 16s rRNA,O157 antigen (rfbE),Vero toxin 1(vt1),Vero toxin 2(vt2) and intimin(eaeA). 目的根据GenBank中编码大肠杆菌16SrRNA的基因、rfbE(O157抗原基因)、vt1(Vero毒素1基因)、vt2(Vero毒素2基因)和eaeA(紧密素基因)5种基因的特异性序列,设计合成5对引物,建立了快速鉴定大肠杆菌O157的多重PCR方法。
Method Acoording to the analysis of the conservative and variable regions in bacterial 16S rRNA genes , we designed universal primers for all bacteria and specific primers for most gram-positive and gram-negtive bacteria . 方法通过对多数病原菌16S rRNA基因保守区和变异区的序列分析，设计通用引物和革兰阴性菌及革兰阳性菌的特异性引物分别作为外侧和内侧引物，建立了对不同细菌的16S rRNA基因进行多重半套式PCR扩增方法；
The genetic diversities of cytochrome b, COI, ND2, ND4, 12S rRNA and 16S rRNA genes of mtDNA were analyzed by PCR-SSCP method among these individuals, and variable sites were found through sequencing analysis which led to the diversities. 通过PCR-SSCP 及DNA 测序的方法对这些品种(共468 个体)线粒体DNA 编码区的Cytb、COI、ND2、ND4、12S rRNA 和16SrRNA 基因设计特异性引物进行多态性分析,并通过测序分析找出了导致多态的变异位点。
The genomic DNA was isolated and purified with the improved CTAB methods. The sequences of 16S rRNA gene were amplified by TD-PCR with the bacterium universal primers and the purified PCR products were directly sequenced. 菌株经纯化培养;以改良CTAB法提取总DNA;采用细菌16S rRNA通用引物、TD-PCR方法(touchdown-PCR)进行16S rRNA基因序列扩增;PCR产物纯化后直接进行序列测定;序列经人工校对后用Clustal X进行比对分析;最后用MEGA3.;1软件构建系统发育树。
To detect the variability and its pathogenecity of Helicobacter pylori(H.pylori),fifty- two biopsy specimens and 18 strains of H.pylori were performed PCR for 16S rRNA gene and flagellin A gene(flaA). PCR-RFLP and PCR-RFLP-SSCP were also performed. 我们对52例胃粘膜活检标本和18株 Hp 临床分离株进行 Hp 特异的16S rRNA 基因和鞭毛素 A 基因 PCR 扩增和 PCR-RFLP 及 PCR-RFLP-SSCP 分析,以检测 Hp 鞭毛素 A 基因的变异性,进而研究其与 Hp 致病性的关系。
Blanchard A, Yanez A, Dybvig K, et al.Evaluation of intraspecies genetic variation within the 16S rRNA gene mycoplasmas homini and detecting by polymerase chainreaction.J Clin Microbiol, 1993,31:1358. 骆丹;黄澍杰;谢礼豪;等.;解脲支原体对七种抗菌药物的敏感性测定及其与相关耐药基因检测的比较
Keywords 16S rDNA;microbial desulfurization;clone; 微生物脱硫;克隆;
Keywords Sequence comparisons;Stem-loop structured probe;16S rRNA;Detection; 序列比对;茎环结构探针;16S核糖体核糖核酸;检测;